Document Type

Article

Publication Date

1-15-2018

Publication Title

Food Chemistry

Abstract

In this study, a novel galactomannoglucan named as TJ2 was isolated from Agaricus brasiliensis with microwave extraction, macroporous resin, ion exchange resin and high resolution gel chromatography. TJ2 is composed of glucose, mannose and galactose in the ratio 99.2:0.2:0.6. Infrared spectra (IR), methylation analysis and nuclear magnetic resonance spectra indicated that TJ2 mainly contained a b-(1?3) – linked glucopyranosyl backbone. Interestingly, TJ2 significantly promoted RAW264.7 cell proliferation, and was able to activate the cells to engulf E. coli. In addition, TJ2 induced the expression of Interleukin 1b (IL-1b), Interleukin 6 (IL-6), tumor necrosis factor a (TNF-a) and cyclooxygenase-2 (Cox-2) in the cells. TJ2 also promoted the production of nitric oxide (NO) by inducing the expression of inducible nitric oxide synthase (iNOS). Moreover, TJ2 is a potent inducer in activating the mitogen-activated protein kinase (MAPK) and inhibitor of nuclear factor-kappa B (IkappaB)/nuclear factor-kappa B (NFkappaB) pathways.

Comments

This research was financially supported by ‘‘131” first level innovative talents training project in Tianjin, Important Project Foundation of Tianijin (No. 12ZCZDNC01400) and Tianjin Thousand Talents program (2013-Zhou).

https://www.sciencedirect.com/science/article/pii/S0308814617311342?via%3Dihub

DOI

10.1016/j.foodchem.2017.06.152

Version

Postprint

Volume

239

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