CSUOH0901, a novel anticancer derivative of nimesulide, exhibits very promising anticancer activities in various cancer cell lines. In order to support further pharmacological and toxicological studies of this promising anticancer drug candidate, an LC-MS/MS method was developed and validated in accordance with the US Food and Drug Administration guidelines. The drug molecules were extracted from plasma samples by protein precipitation and then analyzed with LC-ESI-MS/MS. An excellent analyte separation was achieved using a phenomenex C18 column with a mobile phase of 90% methanol and 5 m m of ammonium formate. The validated linear dynamic range was between 0.5 and 100 ng/mL and the achieved correlation coefficient (r(2)) was >0.9996. The results of inter- and intra-day precision and accuracy were satisfactory, that is,
Voggu, R. R.; Alagandula, R.; Zhou, X.; Su, B.; Zhong, B.; Guo, B. A rapid LC-MS/MS method for quantification of CSUOH0901, a novel antitumor agent, in rat plasma. Biomedical Chromatography 2015, 29, 797-802.
This is the accepted version of the following article:Voggu, R. R.; Alagandula, R.; Zhou, X.; Su, B.; Zhong, B.; Guo, B. A rapid LC-MS/MS method for quantification of CSUOH0901, a novel antitumor agent, in rat plasma. Biomedical Chromatography 2015, 29, 797-802., which has been published in final form at https://onlinelibrary.wiley.com/doi/full/10.1002/bmc.3365