Chemoselective Immobilization of Biomolecules Through Aqueous Diels-Alder and PEG Chemistry

Document Type

Article

Publication Date

4-14-2008

Publication Title

Tetrahedron Letters

Abstract

Aqueous Diels–Alder chemistry combined with a poly(ethylene glycol) (PEG) spacer was used to immobilize a diverse group of biomolecules onto a solid surface. Briefly, α, ω linear PEG conjugates were synthesized containing cyclopentadiene in the α position and either biotin, lactose, or protein A in the ω position. Linkers were coupled to N-maleimide (EMC)-functionalized glass substrates, and surface immobilization of biomolecules was confirmed by confocal fluorescence imaging.

Comments

We acknowledge financial support under the grants from the AHA-0565287B and the AHAF-H2007027 (X.-L.S.).

DOI

10.1016/j.tetlet.2008.02.111

Volume

49

Issue

16

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