Chemoselective Immobilization of Biomolecules Through Aqueous Diels-Alder and PEG Chemistry
Aqueous Diels–Alder chemistry combined with a poly(ethylene glycol) (PEG) spacer was used to immobilize a diverse group of biomolecules onto a solid surface. Briefly, α, ω linear PEG conjugates were synthesized containing cyclopentadiene in the α position and either biotin, lactose, or protein A in the ω position. Linkers were coupled to N-maleimide (EMC)-functionalized glass substrates, and surface immobilization of biomolecules was confirmed by confocal fluorescence imaging.
Sun, X.; Yang, L.; Chaikof, E. L. Chemoselective immobilization of biomolecules through aqueous Diels–Alder and PEG chemistry. Tetrahedron Lett. 2008, 49, 2510-2513.