Document Type
Article
Publication Date
4-2002
Publication Title
Nucleic Acids Research
Abstract
The RNA-recognition motif (RRM) is a common and evolutionarily conserved RNA-binding module. Crystallographic and solution structural studies have shown that RRMs adopt a compact α/β structure, in which four antiparallel β-strands form the major RNA—binding surface. Conserved aromatic residues in the RRM are located on the surface of the β-sheet and are important for RNA binding. To further our understanding of the structural basis of RRM—nucleic acid interaction, we carried out a high resolution analysis of UP1, the N-terminal, two-RRM domain of heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1), whose structure was previously solved at 1.75–1.9 Å resolution. The two RRMs of hnRNP A1 are closely related but have distinct functions in regulating alternative pre-mRNA splice site selection. Our present 1.1 Å resolution crystal structure reveals that two conserved solvent-exposed phenylalanines in the first RRM have alternative side chain conformations. These conformations are spatially correlated, as the individual amino acids cannot adopt each of the observed conformations independently. These phenylalanines are critical for nucleic acid binding and the observed alternative side chain conformations may serve as a mechanism for regulating nucleic acid binding by RRM-containing proteins.
Repository Citation
Vitali, Jacqueline; Ding, Jianzhong; Jiang, Jianzhong; Zhang, Ying; Krainer, Adrian R.; and Xu, Rui-Ming, "Correlated Alternative Side Chain Conformations in the RNA-recognition Motif of Heterogeneous Nuclear Ribonucleoprotein A1" (2002). Physics Faculty Publications. 176.
https://engagedscholarship.csuohio.edu/sciphysics_facpub/176
Original Citation
Jacqueline Vitali et al., "Correlated alternative side chain conformations in the RNA-recognition motif of heterogeneous nuclear ribonucleoprotein A1," Nucleic Acids Res. 30 (7), 1531-1538 (2002).
Publisher's Statement
The advent of online publication has greatly improved access to scientific content on a global scale. This has led to calls from the academic community for research to be made freely available online immediately upon publication, without the barrier of paid subscription to access. In response to these calls, and following consultation with journal contributors, Oxford University Press and the Editors of Nucleic Acids Research (NAR) launched an Open Access initiative for NAR in 2005. This means that it is no longer necessary to hold a subscription in order to read current NAR content online. There are substantial costs associated with publishing a high quality journal such as NAR, for example in the administration of the editorial process, production of the published version, and development of online functionality. Under a subscription-based model, these costs are primarily covered by charging libraries and individuals for access to the journal's content. Under NAR's Open Access model, we aim to cover the costs of publication primarily through a combination of author charges and institutional payments.
Volume
30
Issue
7
