Exploring the Effect of Preferential Hydration on the Dynamics of Lysozyme in Glycerol-Water Solutions

Document Type

Conference Proceeding

Publication Date

3-16-2010

Publication Title

APS March Meeting

Abstract

The relaxation processes of the protein lysozyme in glycerol-water solutions were studied with Dielectric Spectroscopy and Dynamic Light Scattering (DLS) in a wide temperature range. Analysis of the dielectric spectra revealed three relaxation processes: protein rotation, large scale protein motions (slow δ-relaxation), and solvent α-relaxation. Analysis reveals a decoupling of protein dynamics from the solvent relaxation. These effects were also studied by comparing the protein diffusion as measured by DLS and the viscosity of solutions. Both spectroscopic techniques suggest preferential hydration of the protein in solution.

Volume

55

Issue

2

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